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Diagnostic Automation, Inc.
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Free Estriol (antigen) in the sample compets with horseradish
peroxidase-Estriol (enzyme-labeled antigen) for binding onto
the limited number of anti-Estriol(antibody) sites on the
microplates (solid phase).
After incubation the bound/free separation is performed by a
simple solid-phase washing.
The enzyme substrate (H2O2) and the TMB-Substrate (TMB)
are added. After an appropriate time has elapsed for maximum
color development, the enzyme reaction is stopped and the
absorbance are determinated.
Estriol concentration in the sample is calculated based on a
series of standard.
The color intensit
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