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ALTERNATE VOLUMES
If the spectrophotometer being used can accurately read the final
volume of 0.50 ml or smaller, follow the "ALTERNATE
PROCEDURE".
Unit: One unit is the amount of enzyme that catalyzes the
transformation of one micromole of substrate per minute under the
specified conditions.
IU/L = Δ Abs./ min. × 1000 × TV = Δ Abs./min. × 1000 × 1.025
∈ × LP × SV 18.75 × 1 × 0.025
= Δ Abs./min. x 2187
Where:
Δ Abs./min = Absorbance change
1000 = Conversion of IU/ml to IU/L
TV = Total reaction volume (1.025 ml)
∈ = Millimolar absorptivity of p-Nitrophenol 18.75
LP = Light path in centimeters (
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